ht-29 cells Search Results


human colorectal adenocarcinoma cells ht 29  (CLS Cell Lines Service GmbH)
94
CLS Cell Lines Service GmbH human colorectal adenocarcinoma cells ht 29
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Human Colorectal Adenocarcinoma Cells Ht 29, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/HT-29+Cells/pmc12689631-247-0-5
Average 94 stars, based on 1 article reviews
human colorectal adenocarcinoma cells ht 29 - by Bioz Stars, 2026-09
94/100 stars
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ht 29 cancer cells  (Genecopoeia)
94
Genecopoeia ht 29 cancer cells
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Ht 29 Cancer Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/Human+cell+line+HT-29+stably+expressing+CRISPR+Cas9%2C+randomly+inserted%2C+single+clone/pmc06978517-210-5-16
Average 94 stars, based on 1 article reviews
ht 29 cancer cells - by Bioz Stars, 2026-09
94/100 stars
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ht29  (OriGene)
93
OriGene ht29
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Ht29, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/HT29+(Human+Colon+Colorectal+Adenocarcinoma)+Whole+Cell+Lysate/10__1158_slash_1078___0432__ccr___11___1877-57-4-18
Average 93 stars, based on 1 article reviews
ht29 - by Bioz Stars, 2026-09
93/100 stars
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ht 29 egfp  (Genecopoeia)
94
Genecopoeia ht 29 egfp
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Ht 29 Egfp, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/HT-29+human+colorectal+adenocarcinoma+cancer+cell+line+labeled+with+GFP/pm32210258-197-0-5
Average 94 stars, based on 1 article reviews
ht 29 egfp - by Bioz Stars, 2026-09
94/100 stars
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cell lines  (Korean Cell Line Bank)
86
Korean Cell Line Bank cell lines
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Cell Lines, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/29+ht/pm40907592-50-12-18
Average 86 stars, based on 1 article reviews
cell lines - by Bioz Stars, 2026-09
86/100 stars
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ht29-mtx e12 cells  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures ht29-mtx e12 cells
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Ht29 Mtx E12 Cells, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/ht29+mtx+e12+cells/pm31139862-59-4-13
Average 90 stars, based on 1 article reviews
ht29-mtx e12 cells - by Bioz Stars, 2026-09
90/100 stars
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ht-29-luc-d6 bioware® cell line  (BioWare Corporation)
90
BioWare Corporation ht-29-luc-d6 bioware® cell line
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Ht 29 Luc D6 Bioware® Cell Line, supplied by BioWare Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/ht29+luc+d6+human+colon+cancer+cells/pm24704711-138-16-19
Average 90 stars, based on 1 article reviews
ht-29-luc-d6 bioware® cell line - by Bioz Stars, 2026-09
90/100 stars
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ht-29 cells  (SMAC Corp)
90
SMAC Corp ht-29 cells
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Ht 29 Cells, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/ht+29+cells/pmc08379748-151-110-38
Average 90 stars, based on 1 article reviews
ht-29 cells - by Bioz Stars, 2026-09
90/100 stars
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ht-29 cells constitutively expressing mlkl fused c-terminally to the str2-3xflag tag  (Gloeckner Foundation)
90
Gloeckner Foundation ht-29 cells constitutively expressing mlkl fused c-terminally to the str2-3xflag tag
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Ht 29 Cells Constitutively Expressing Mlkl Fused C Terminally To The Str2 3xflag Tag, supplied by Gloeckner Foundation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/ht+29+cells+constitutively+expressing+mlkl+fused+c+terminally+to+the+str2+3xflag+tag/pm28666573-276-25-27
Average 90 stars, based on 1 article reviews
ht-29 cells constitutively expressing mlkl fused c-terminally to the str2-3xflag tag - by Bioz Stars, 2026-09
90/100 stars
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human colorectal cancer cell line ht-29 cpq-57  (ProQinase GmbH)
90
ProQinase GmbH human colorectal cancer cell line ht-29 cpq-57
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Human Colorectal Cancer Cell Line Ht 29 Cpq 57, supplied by ProQinase GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/human+colorectal+cancer+cell+line+ht+29+cpq+57/us11298359-1510-5-12
Average 90 stars, based on 1 article reviews
human colorectal cancer cell line ht-29 cpq-57 - by Bioz Stars, 2026-09
90/100 stars
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ht-29 cells  (DS Pharma Biomedical)
90
DS Pharma Biomedical ht-29 cells
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Ht 29 Cells, supplied by DS Pharma Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/ht+29+cells/pmc10108455-39-0-5
Average 90 stars, based on 1 article reviews
ht-29 cells - by Bioz Stars, 2026-09
90/100 stars
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human intestinal epithelial ht29 cells  (CH Instruments)
90
CH Instruments human intestinal epithelial ht29 cells
a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines <t>RD-ES,</t> <t>HT-29</t> and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.
Human Intestinal Epithelial Ht29 Cells, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht-29+cells/ht+29+cells/pm27107253-103-25-30
Average 90 stars, based on 1 article reviews
human intestinal epithelial ht29 cells - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines RD-ES, HT-29 and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.

Journal: Nature Communications

Article Title: Search-and-remove genome editing allows selection of cells by DNA sequence

doi: 10.1038/s41467-025-66896-1

Figure Lengend Snippet: a Strategy to apply SNIPE to kill cancer cells based on cancer specific mutations. Wild type cells are colored in gray, cells with cancer mutations in purple and dying cells in red. b Cell growth curves of human 409B2 iPSCs measured by resazurin assay (upper panel). The lower panel shows NGS read frequency for cancer mutation (purple) and wild type cells (gray) in a mixed population over time. c Killing of TP53 knock-out 409B2 cells by SNIPE. Different amounts of TP53 knock-out cells (purple) were mixed with wild type cells (gray). Indels are colored in blue and wild type with indels in light purple. d Cell survival after selection by SNIPE related to ( c ). e K562 cells carry a Philadelphia chromosome, characterized by a reciprocal translocation of chromosome 9 and 22 resulting in the fusion oncogene BCR-ABL1 . f The BCR-ABL fusion generates a new gRNA recognition site in K562 cells. g Cell survival of THP-1 and K562 cells after targeting the BCR-ABL junction. The label “Cas9 cut” indicates CRISPR targeting without SNIPE. h Agarose gel of PCR products of healthy chromosome 22 (297 bp, gray) and Philadelphia chromosome (267 bp, purple). i Quantification of band intensity for K562 cells of ( h ). j Cell survival of heterozygous cancer cell lines RD-ES, HT-29 and HuCC-T1 after targeting the disease allele with SNIPE. k NGS read frequency of cancer locus alleles in RD-ES, HT-29 and HuCC-T1 for mock and SNIPE edit. The cancer mutation allele is colored in purple, wild type in grey, indels in blue and wild type with indels in light purple. Independent biological replicates were performed ( n = 3 for b , c , d , g , h , i , j , k , n = 2 for RDES in j and k) and are depicted as dots for the cancer mutation allele sequence reads and cell survival. Error bars show the s.e.m.

Article Snippet: Human colorectal adenocarcinoma cells HT-29 (Cytion, catalog no. 300215) were cultured in EMEM (Sigma-Aldrich, catalog no. M4655) supplemented with 10% FBS (Gibco, catalog no. 10270-106) and human CML cells K562 (ECACC, catalog no. 89121407) were cultured in IMDM (Gibco, catalog no. 12440053) with 10% FBS.

Techniques: Resazurin Assay, Mutagenesis, Knock-Out, Selection, Translocation Assay, CRISPR, Agarose Gel Electrophoresis, Sequencing